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Evaluating the effect of ultrasmall superparamagnetic iron oxide nanoparticles for a long-term magnetic cell labeling
Saeed Shanehsazzadeh1, Mohammad Ali Oghabian1, Barry J Allen2, Massoud Amanlou3, Afshin Masoudi4, Fariba Johari Daha5
1 Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran, Iran
2 Center for Experimental Radiation Oncology, Cancer Care Center, St George Hospital, Kogarah, NSW, Sydney, Australia
3 Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
4 Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran
5 Radioisotope Division, Nuclear Research Center, Atomic Energy Organization of Iran, Tehran, Iran
Correspondence Address:
Mohammad Ali Oghabian
Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0971-6203.106603
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In order to evaluate the long-term viability, the iron content stability, and the labeling efficiency of mammalian cells using magnetic cell labeling; dextran-coated ultrasmall superparamagnetic iron oxide (USPIOs) nanoparticles with plain surfaces having a hydrodynamic size of 25 nm were used for this study. Tests were carried out in four groups each containing 5 flasks of 5.5 × 10 6 AD-293 embryonic kidney cells. The cell lines were incubated for 24 h using four different iron concentrations with and without protamine sulfate (Pro), washed with phosphate-buffered saline (PBS) and centrifuged three times to remove the unbounded USPIOs. Cell viability was also verified using USPIOs. There were no significant differences in the cell viability between the control group of cells and those groups with iron uptake at the specified iron concentrations. The average iron uptake ratio compared to that of the control group was (114 ± 1). The magnetic resonance images (MRI) at post-labeling day 1 and day 21 showed (75 ± 4)% and (22 ± 5)% signal decrements compared to that of the control, respectively. The Perl's Prussian blue test showed that 98% of the cells were labeled, and the iron concentration within the media did not affect the cell iron uptake. Magnetic cellular labeling with the USPIO-Pro complex had no short or medium term (3 weeks) toxic effects on AD-293 embryonic kidney cells. |
